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| 珍稀濒危植物珙桐种子休眠萌发过程中内源激素的变化 |
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雷泞菲1,2, 彭书明1,2, 牛 蓓1, 周嘉峪1, 苏智先3, 陈 放1*
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1.四川大学 生命科学学院, 成都 610041;2.成都理工大学 材料与化学
化工学院, 成都 610059;3.绵阳师范学院, 四川 绵阳 621000
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| 摘要: |
| 珙桐是我国特有珍稀濒危植物,休眠期长且具二次休眠现象。将处于休眠萌发过程中的珙桐种子依据胚根长度划分为4个阶段,利用高效液相色谱(HPLC)测定各阶段种子及其内果皮中ABA(脱落酸)、GA(赤霉素)、KT(细胞分裂素)、IAA(3-吲哚乙酸)4种内源激素含量,分析其比值动态变化,并与幼苗阶段进行比较。结果显示:未破壳种子的内果皮中内源激素含量以ABA最高,其次是GA、IAA、KT,随着种子破壳后四种激素含量显著降低。除ABA外,种子中GA、IAA和KT含量随着胚根的伸长逐渐升高,但仍低于幼苗阶段。此外,随着胚根伸长,种子中GA/ABA、IAA/ABA、KT/ABA比值逐渐增大,其中以GA/ABA的变化最显著。因此,珙桐种子的休眠和萌发可能主要受ABA和GA的平衡和拮抗来调控。 |
| 关键词: 珙桐 内源激素 休眠 萌发 |
| DOI： |
| 分类号:Q945 |
| Fund project: |
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| Changes on the edogenous phytohormone content of the Davidia involucrate seed during dormancy and germination process |
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LEI Ning-Fei1,2, PENG Shu-Ming1,2, NIU Bei1,
ZHOU Jia-Yu1, SU Zhi-Xian3, CHEN Fang1*
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1.College of Life Science, Sichuan University, Chengdu 610041, China;2.College of Materials
and Chemistry &3.Chemical Engineering, Chengdu University of Technology, Chengdu
610059, China;4.3.Mianyang Teachers College, Mianyang 621000, China
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| Abstract: |
| Davidia involucrate is an ornamental tree native to China and one of the very rare and endangered plants,which has double dormancy as well as a long dormant period. In the study,dormancy and germination process of Davidia involucrate seeds was divided into four stages according to radicle length. The changes of ABA(Abscisic acid),IAA(Indole-3-acetic acid),GA(Gibberellin)and KT(Kinetin)content in seeds and endocarp were determined by using HPLC among four stages. In addition,the dynamics on GA/ABA,IAA/ABA,KT/ABA and KT/IAA ratio were analysed among four stages. Finally,above-mentioned data were compared with those in seedlings stage. The results showed that:(1)the endogenous hormones content order is ABA>GA>IAA>KT in endocarp of uncracked seeds,the four endogenous hormones content decreased significantly after seeds were cracked;(2)except ABA,GA,IAA and KT contents in seeds increased gradually with radicle elongation. However,the GA,IAA and KT contents were less compared to those in seedlings;(3)in addition,GA/ABA,IAA/ABA and KT/ABA ratio in seed increased gradually with radicle elongation. The change in GA/ABA ratio was greatest than the other two ones. So it is suggested that the trade-off between ABA and GA contents could control the dormancy and germination process in Davidia involucrate seed. |
| Key words: Davidia involucrate phytohormones dormancy germination |
