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| 苦丁茶冬青不同种质材料的酯酶同工酶分析 |
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郑道君1,2, 刘国民1,2*, 何声进2, 李娟玲1,2
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1.海南大学 热带生物资源教育部重点实验室, 海口 570228;2.海南大学 苦丁茶研究所, 海口 570228
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| 摘要: |
| 应用聚丙烯酰胺凝胶电泳比较了苦丁茶冬青共50份种质材料酯酶同工酶的酶谱差异,计算了供试种质材料间的Jaccard相似性系数,并运用UPGMA法进行聚类分析。研究结果表明,供试的50份苦丁茶冬青种质材料共显示出其13条谱带,材料间的酯酶同工酶酶谱差异显著,其具体表现在迁移率、酶带数目和酶带强弱的显著不同,呈现出丰富的多态性。基于酯酶酶谱的50份供试种质材料间的相似系数变化范围在0.3000～1.0000之间,UPGMA法聚类结果与不同种质材料的地理起源有显著的相关性。聚类结果可以把不同地域起源的材料分开,而同一地域来源的材料则归聚在一起。酯酶同工酶的分析结果可以作为评价苦丁茶冬青种内遗传多样性以及种下类群划分的重要参数之一。 |
| 关键词: 苦丁茶冬青 酯酶同工酶 遗传多样性 聚类分析 |
| DOI： |
| 分类号:Q943 |
| Fund project: |
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| Esteras isoenzymes analysis of different germplasm materials of Ilex kudingcha |
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ZHENG Dao-Jun1,2, LIU Guo-Min1,2*, HE Sheng-Jin2, LI Juan-Ling1,2
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1.Key Laboratory of Tropical Biological Researches, Ministry of Education, Hainan University, Haikou
570228, China;2.Kudingcha Research Institute, Hainan University,Haikou 570228, China
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| Abstract: |
| The difference of esteras isoenzymes of 50 germplasm materials of Ilex kudingcha was compared in this thesis by means of polyacrylamid gel electrophoresis. Jaccard similarity coefficient of 50 germplasm materials was calculated,and the UPGMA was used for cluster analysis. The results showed that there were 13 esteras isozymes bands observed in all test materials,esteras isoenzymes of all test germplasm materials were obviously different,which embodied on the ratio of flow(Rf),the number of esteras isoenzymes bands and strong or weak of the bands,which showed abundant polymorphism for I.kudingcha. The similarity coefficients among the test materials based on esteras isozymes ranged from 0.3000-1.0000; UPGMA cluster result had batter correlation with the origins of germplasm materials,which could distinguish the germplasm materials from different origin and cluster the germplasm materials from the same origin together. The analysis results of esteras isozymes could be considered as one of the significant references for assessing the genetic diversity and the classification of infra-specific taxa of I.kudingcha. |
| Key words: Ilex kudingcha esteras isoenzymes genetic diversity cluster analysis |
