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| 防风地上部分化学成分及抗炎活性研究 |
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刘振强1, 周淑鑫1, 周露瑶1, 林宇轩1, 刘 爽1, 张 倩1, 孙 艳1, 陈庆山2,
张丽莉2, 匡海学1, 刘 艳1,杨炳友1*
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1. 黑龙江中医药大学 药学院, 教育部北药基础与应用研究重点实验室, 哈尔滨 150040;2. 东北农业大学 农学院, 哈尔滨 150030
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| 摘要: |
| 为研究防风(Saposhnikovia divaricata)地上部分的抗炎活性成分,该研究采用硅胶、ODS等柱色谱方法以及高效液相技术对70%乙醇提取物进行分离纯化,并结合理化性质及1H-NMR、13C-NMR、MS等波谱学数据对所分离化合物进行结构鉴定,采用脂多糖(LPS)诱导的RAW 264.7细胞模型测定化合物对一氧化氮(NO)的抑制活性。结果表明:(1)从防风地上部分中分离得到15个化合物,分别鉴定为姜糖脂A(1),(E)-2-己烯基-O-β-D-吡喃葡萄糖苷(2),(Z)-3-己烯基-O-β-D-吡喃葡萄糖苷(3),正己烷基-O-β-D-吡喃葡萄糖苷(4),sachalinoside B(5),5β,6α-dihydroxy-3β-(β-D-glucopyranosyloxy)-7-megastigmen-9-one(6),phenethyl-β-D-glucopyranoside(7),没食子酸乙酯(8),香草酸(9),蚱蜢酮(10),2-乙氧基-2-对羟基苯基乙醇(11),2-methoxy-2-(4'-hydroxyphenyl)ethanol(12),1,2,3,4,6-penta-O-gally -β-D-glucopyranose(13),(-)-当归棱子芹醇-2-O-β-D-呋喃芹菜糖基-(1→6)-β-D-吡喃葡萄糖苷(14),(9Z,12Z)-N-(2-hydroxyethyl)octadeca-9,12-dienamide(15)。其中,化合物1-5、7-10首次在伞形科植物中分离得到,化合物11-15首次从防风属植物中发现。(2)对化合物1-15进行了体外抗炎活性实验,结果表明化合物1、3、4、7、9、12、14对LPS诱导的RAW 264.7细胞NO释放具有不同程度的抑制作用。 |
| 关键词: 防风, 地上部分, 化学成分, 提取分离, 结构鉴定, 抗炎活性 |
| DOI：10.11931/guihaia.gxzw202408047 |
| 分类号:Q946 |
| 文章编号:1000-3142(2025)02-0207-10 |
| Fund project:国家自然科学基金区域联合创新基金重点项目(U22A20370); 黑龙江省重点研发计划项目(GA21D008); 黑龙江省中医药管理局中医药科研课题项目(ZHY2024-001); 黑龙江省“双一流”学科协同创新成果建设项目(LJGXCG2022-096)。 |
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| Investigation on chemical components and anti-inflammatory activities from the aerial parts of Saposhnikovia divaricata |
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LIU Zhenqiang1, ZHOU Shuxin1, ZHOU Luyao1, LIN Yuxuan1, LIU Shuang1, ZHANG Qian1,
SUN Yan1, CHEN Qingshan2, ZHANG Lili2, KUANG Haixue1, LIU Yan1, YANG Bingyou1*
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1. Key Laboratory of Basic and Application Research of Northern Medicine of Ministry of Education, College of Pharmacy Heilongjiang University
of Chinese Medicine, Harbin 150040, China;2. College of Agriculture, Northeast Agricultural University, Harbin 150030, China
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| Abstract: |
| In order to investigate chemical components and anti-inflammatory activities from the aerial parts of Saposhnikovia divaricata. Silica gel, ODS and HPLC, etc. were utilized for the separation and purification of the 70% ethanol extract. The structures of the isolated compounds were characterized by integrating physicochemical properties and spectroscopic data including 1H-NMR, 13C-NMR, and MS. Their inhibitory activity of the compounds on nitric oxide(NO)was determined by lipopolysaccharide(LPS)-induced RAW 264.7 cell model. The results were as follow:(1)Fifteen compounds were isolated from the aerial parts of Saposhnikovia divaricata. They were identified as gingerglycolipid A(1),(E)-2-hexenyl-O-β-D-glucopyranoside(2),(Z)-3-hexenyl-O-β-D-glucopyranoside(3), hexyl-O-β-D-glucopyranoside(4), sachalinoside B(5), 5β,6α-dihydroxy-3β-(β-D-glucopyranosyloxy)-7-megastigmen-9-one(6), phenethyl-β-D-glucopyranoside(7), ethylgallate(8), vanillic acid(9), grasshopper ketone(10), 2-ethoxy-2-(4-hydroxyphenyl)-ethanol(11), 2-methoxy-2-(4'-hydroxyphenyl)ethanol(12), 1,2,3,4, 6-penta-O-gally-β-D-glucopyranose(13),(-)-angelica angellinol-2-O-β-D-furan celery glycosyl-(1→6)-β-D-glucopyranoside(14),(9Z,12Z)-N-(2-hydroxyethyl)octadeca-9,12-dienamide(15). Among them, compounds 1-5 and 7-10 were isolated from plants of the Umbelliferae for the first time, and compounds 11-15 were discovered from plants of the Saposhnikovia genus for the first time.(2)In vitro anti-inflammatory activity experiments were conducted on compounds 1-15. The results showed that compounds 1, 3, 4, 7, 9, 12 and 14 could inhibit the release of NO from RAW 264.7 cells induced by LPS. |
| Key words: Saposhnikovia divaricata, aerial part, chemical components, extraction and separation, structural identification, anti-inflammatory activity |
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