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| 石韦不同极性萃取物体外降血糖活性研究 |
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庄远杯1,2, 凌梅娣2, 魏爱红1,2, 李榕娣1,2, 刘小敏1,2, 聂 华1,2, 张声源1,2*
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1. 嘉应学院 广东省山区特色农业资源保护与精准利用重点实验室, 广东 梅州 514015;2. 嘉应学院 医学院 客家药用生物资源研究所, 广东 梅州 514031
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| 摘要: |
| 为进一步明确石韦对α-葡萄糖苷酶和α-淀粉酶抑制作用机制,该研究以石韦95%乙醇提取物的不同极性萃取物为试材,阿卡波糖为阳性对照,采用pNPG法(p-nitrophenyl-α-D-glucopyranoside, pNPG)、DNS法(3,5-dinitro salicylic acid, DNS)考察石韦不同极性萃取物的α-葡萄糖苷酶和α-淀粉酶抑制活性,并采用酶促动力学方法与Lineweaver-Burk曲线分析最强活性萃取物对α-葡萄糖苷酶的抑制作用类型,以期为石韦的进一步开发利用提供科学依据。结果表明:石韦水萃取物对α-葡萄糖苷酶和α-淀粉酶的抑制作用最强,其IC50值分别为(4.71 ± 0.72)μg·mL-1、IC50 =(48.40 ± 0.32)μg·mL-1,并显著强于其他萃取物(P<0.05),且对α-葡萄糖苷酶抑制作用比阿卡波糖强,对α-淀粉酶抑制作用弱于阿卡波糖。阿卡波糖对α-葡萄糖苷酶和α-淀粉酶抑制作用的IC50值分别为(2 857.36 ± 1.35)μg·mL-1和(16.41 ± 0.63)μg·mL-1。酶促动力学显示水萃取物对α-葡萄糖苷酶为可逆性抑制,Lineweaver-Burk曲线显示为竞争性抑制。综上结果表明,石韦水萃取物具有较好的α-葡萄糖苷酶和α-淀粉酶抑制活性,是一种胃肠道副作用小、天然α-葡萄糖苷酶抑制剂来源。 |
| 关键词: 石韦, α-葡萄糖苷酶, α-淀粉酶, 阿卡波糖, 提取物 |
| DOI：10.11931/guihaia.gxzw202008063 |
| 分类号:Q946 |
| 文章编号:1000-3142(2022)05-0855-05 |
| Fund project:国家自然科学基金青年基金(81703662); 广东省科技计划项目(2020B121201013); 梅州市医药卫生科研课题(2019-B-10); 梅州市社会发展科技计划项目(2018B057); 广东省高等教育“冲补强” 提升计划重点建设学科(农业资源与环境)建设项目(粤教科函[2018]181号); 广东省大学生创新创业项目(201910582267)[Supported by National Natural Science Foundation of China(81703662); Science and Technology Project of Guangdong Province(2020B121201013); Medical and Health Research Project of Meizhou(2019-B-10); Social Development Science and Technology Project of Meizhou(2018B057); Promotion Project of Higher Education in Guangdong Province about Key Construction Subjects(Agricultural Resources and Environment)(Guangdong Science and Education Letter [2018]181); Guangdong University Student Innovation and Entrepreneurship Project(201910582267)]。 |
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| Hypoglycemic activities of different solvent extracts from Pyrrosia lingua in vitro |
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ZHUANG Yuanbei1,2, LING Meidi2, WEI Aihong1,2, LI Rongdi1,2,
LIU Xiaomin1,2, NIE Hua1,2, ZHANG Shengyuan1,2*
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1. Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous
Areas, Jiaying University, Meizhou 514015, Guangdong, China;2. Institute of Hakka Medicinal Bio-resources, Medical
College, Jiaying University, Meizhou 514031, Guangdong, China
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| Abstract: |
| In order to further make clear the inhibition mechanism of Pyrrosia lingua on α-glucosidase and α-amylase, their inhibitory activities of different polar extracts of 95% ethanol extracts of P. lingua were investigated by using p-nitrophenyl-α-D-glucopyranoside(pNPG)and 3,5-dinitro salicylic acid(DNS)methods with acarbose as positive control. Enzyme kinetics method and Lineweaver-Burk curve were used to analyze the types of inhibition of α-glucosidase for the most active extract. The results showed that the IC50 values of the water extract were(4.71 ± 0.72)μg·mL-1 for α-glucosidase and(48.40 ± 0.32)μg·mL-1 for α-amylase respectively, which were significantly stronger than those of the other extracts(P < 0.05). The inhibition of α-glucosidase was stronger than that of acarbose, while the inhibition of α-amylase was weaker than that of acarbose. The IC50 values of acarbose on α-glucosidase and α-amylase were(2 857.36 ± 1.35)μg·mL-1 and(16.41 ± 0.63)μg·mL-1, respectively. The enzymatic kinetics showed that the inhibitory type on α-glucosidase of water extract was reversible inhibition, while the Lineweaver-Burk curve showed a competitive inhibition. In conclusion, the water extract from P. lingua displays potential inhibitory activity on α-glucosidase and α-amylase, can be the natural sources of α-glucosidase inhibitor with less gastrointestinal side effects. |
| Key words: Pyrrosia lingua, α-glucosidase, α-amylase, acarbose, extract |
