| 摘要: |
| 为探究甜茶(Rubus suavissimus)中具有α-葡萄糖苷酶抑制活性的次级代谢产物,该文利用多种现代色谱分离技术对其干燥叶进行提取分离纯化,综合运用质谱、核磁共振波谱分析方法确定了单体化合物的结构,并对分离得到的化合物进行了α-葡萄糖苷酶抑制活性的测试。结果表明:(1)从甜茶的干燥叶中分离鉴定出10个化合物,分别为甜茶苷(1)、山奈酚-3-O-洋槐糖苷(2)、没食子酸(3)、二聚松柏醇(4)、5-甲氧基二聚松柏醇(5)、云实酸(6)、斯替维单糖苷(7)、斯替维醇(8)、16α,17-二羟基对映贝壳杉烷(9)、槲皮素-3-O-β-D-吡喃半乳糖苷(10),其中化合物2、4、5、9均为首次从甜茶中分离得到。(2)α-葡萄糖苷酶抑制活性测试结果显示,化合物2、3、5、6、10具有较强的α-葡萄糖苷酶抑制活性。该研究结果丰富了甜茶中具有α-葡萄糖苷酶抑制活性的化合物,并为降血糖相关产品的开发提供了理论依据。 |
| 关键词: 甜茶, α-葡萄糖苷酶, 悬钩子属, 蔷薇科, 糖尿病 |
| DOI：10.11931/guihaia.gxzw202303011 |
| 分类号:Q946 |
| 文章编号:1000-3142(2024)02-0354-08 |
| Fund project:广西科学院基本科研业务费项目(CQZ-C-1901); 广东省重点领域研发计划项目(2020B1111110003)。 |
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| Chemical constituents from Rubus suavissimus and their α-glucosidase inhibitory activities |
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YANG Bingyuan, HE Ruijie, WANG Yafeng, YAN Xiaojie, HUANG Yonglin*
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Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, Guangxi Institute of Botany, Guangxi
Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China
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| Abstract: |
| Rubus suavissimus is mainly distributed in Guilin, Liuzhou, Wuzhou and other regions in Guangxi Zhuang Autonomous Region, so it is called as “Guangxi tiancha” in China. R. suavissimus, together with Siraitia grosvenorii and Stevia rebaudianastevia are praised as three famous sweet plants in Guangxi Zhuang Autonomous Region. Zhuang and Yao people use the leaves of R. suavissimus as a tea to treat diabetes, and it is known as the “divine tea” of Yao medicine. Therefore, R. suavissimus is a combination of sugar, tea and medicine, which has great potential for the development of food and medicine. In order to investigate the secondary metabolites with α-glucosidase inhibitory activity from R. suavissimus, herein, the extraction, separation and purification of secondary metabolites were performed on the leaves of R. suavissimus. The structures of purified compounds were determined based on the data of mass spectrometry(HR-ESI-MS)and nuclear magnetic resonance(1H NMR and 13C NMR). In addition, the α-glucosidase inhibitory activity of the purified compounds were evaluated by pharmacological methods simultaneously. The results were as follows:(1)Ten compounds were purified and their structures were elucidated as rubusoside(1), kaempferol 3-O-robinobioside(2), gallic acid(3), dihydrodehydroconiferyl alcohol(4), 5-methoxydihydrodehydroconiferyl alcohol(5), brevifolincarboxylic acid(6), steviolmonoside(7), steviol(8), 16α, 17-dihydroxykaurane(9), and quercetin 3-O-β-D-galactopyranoside(10). Among them, compounds 2, 4, 5 and 9 were isolated from R. suavissimus for the first time.(2)Compounds 2, 3, 5, 6 and 10 showed strong inhibitory activity on α-glucosidase. The IC50 values of compounds 2, 3, 5, 6 and 10 were(0.14 ± 0.03)mg·mL-1,(0.36 ± 0.02)mg·mL-1,(0.44 ± 0.01)mg·mL-1,(0.53 ± 0.04)mg·mL-1 and(0.14 ± 0.03)mg·mL-1 respectively, which were stronger than the positive control acarbose with the IC50 values as(0.69 ± 0.02)mg·mL-1. Thus, compounds 2, 3, 5, 6 and 10, which were isolated from the leaves of R. suavissimus, could be a potential α-glucosidase inhibitors based on their bioactivity results. Compounds with α-glucosidase inhibitory activity from R. suavissimus will provide the basis for development of related hypoglycemic products. |
| Key words: Rubus suavissimus, α-glucosidase, Rubus, Rosaceae, diabetes |
