| 摘要: |
| 槲蕨为喀斯特地区典型的附生蕨类植物,主要通过其根茎附生于岩石的表面或缝隙中。槲蕨以干燥的根茎入药,具有重要的药用价值。为探究钙离子(Ca2+)胁迫下槲蕨根茎中代谢物的变化,该研究采用超高效液相色谱联用四极杆飞行时间质谱(UPLC-QTOF/MS)技术对不同浓度(0、600、1 200 mmol·L-1)Ca2+胁迫下槲蕨根茎进行非靶向代谢组学分析。结果表明:(1)共鉴定到64种差异表达代谢物。(2)在0、600 mmol·L-1比较组中有48个差异表达代谢物,在0、1 200 mmol·L-1比较组中有45个差异表达代谢物,在600、1 200 mmol·L-1比较组中有44个差异表达代谢物。(3)鉴定到的差异表达代谢物根据其化学分类归属信息进行归类,分为5类。综上认为,Ca2+胁迫影响槲蕨根茎的氨基酸代谢、黄酮类化合物生物合成、木质素生物合成、脂肪酸代谢及其他途径。通过非靶向代谢组学分析,初步揭示了参与槲蕨根茎应答Ca2+胁迫的关键代谢物,为进一步研究槲蕨适应Ca2+胁迫的调控机制奠定了基础,也为槲蕨根茎药材的品质改善提供了新思路。 |
| 关键词: 非靶向代谢组学, 高浓度Ca2+, 代谢通路, 适应机制, 苗药 |
| DOI：10.11931/guihaia.gxzw202302017 |
| 分类号:Q945.34 |
| 文章编号:1000-3142(2024)03-0531-10 |
| Fund project:国家自然科学基金(31860056)。 |
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| Analysis of differentially expressed metabolites in Drynaria roosii rhizome in response to calcium stress |
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WU Yilin, LI Hui, MA Hongna, LI Weizhong, TAN Longyan*
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School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, China
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| Abstract: |
| Drynaria roosii is a typical epiphytic pteridophyte in Karst area, which lives on the surface or crevice of rocks mainly through its rhizome. The dried rhizome of D. roosii is used as medicine and has important medicinal value. In order to study the changes of metabolites in D. roosii rhizome under Ca2+ stress, the D. roosii rhizome under different concentrations(0, 600, 1 200 mmol·L-1)of Ca2+ stress was analyzed by using the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF/MS). The results were as follows:(1)A total of 64 differentially expressed metabolites were identified.(2)Forty-eight differentially expressed metabolites were identified between the 0 mmol·L-1and 600 mmol·L-1 comparison group, 45 differentially expressed metabolites were identified between the 0 mmol·L-1 and 1 200 mmol·L-1 comparison group, and 44 differentially expressed metabolites were identified between the 600 and 1 200 mmol·L-1 comparison group.(3)The identified differentially expressed metabolites were classified into five categories according to their chemical classification information. In summary, Ca2+ stress affects the amino acid metabolism, flavonoids biosynthesis, lignin biosynthesis, fatty acid metabolism and other pathways of D. roosii rhizome. Through non-targeted metabolomics analysis, the key metabolites that response to Ca2+ stress in D. roosii rhizome are revealed. These results lay a foundation for further study on the regulatory mechanism of D. roosii adaptation to Ca2+ stress, and also provide a new idea for the quality improvement of medicinal materials of D. roosii rhizome. |
| Key words: non-targeted metabolomics, high concentration of Ca2+, metabolic pathway, adaptive mechanism, Miao medicine |
