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葎草多糖含量测定及其抗氧化性研究
王新风1, 杨 芳1,2, 戈群妹1, 陈珊珊1
1.淮阴师范学院 生物系资源微生物研究所, 江苏 淮安 223300;2.南京师范大学 生命科学学院 微生物工程重点实验室, 南京 210097
摘要:
对8月和10月采集的葎草全草、嫩头、叶和茎中多糖含量进行测定,并对各部位提取纯化的多糖进行体外抗氧化作用研究。结果表明,8月份嫩头中多糖含量最高,达到(42.897±2.996)mg/g; 浓度为5 mg/mL的各部位葎草多糖溶液,对Fenton反应生成的羟自由基具有较强的清除能力,达85.15%~98.52%; 对邻苯三酚自氧化法产生的超氧阴离子自由基也有较好的抑制能力,达57.15%~67.54%。葎草多糖对羟自由基和超氧阴离子自由基具有较高的清除能力。
关键词:  葎草  多糖  抗氧化性
DOI:
分类号:Q946.3
Fund project:
Content and antioxidant activity of polysaccharide extracted from Humulus scandens
WANG Xin-Feng1, YANG Fang1,2, GE Qun-Mei1, CHEN Shan-Shan1
1.Institute of Resource Microorganism, Huaiyin Teachers'College, Huaian 22330, China;2.Key Laboratory of Microbial Engineering, College of Life Sciences, Nanjing Normal University, Nanjing 210097, China
Abstract:
The contents of polysaccharide in the whole plant,apic bud,leave and stam of Humulus scandens in August and October,the scavenging effect of its purified polysaccharide on O</sup>-2·and ·OH were investigated.The results showed that the content of polysaccharide in apic bud in August was the highest,reached(42.897±2.996)mg/g. The concentration of 5 mg/mL polysaccharide of H.scanalens can scavenge hydroxyl free radical formed in Fenton raction,and the elimination rate reached 85.15%-98.52%. Oxygen free radicals of self-oxidantion of pyrogallol was well inhibited by the polysaccharide,and the elimination rate reached 57.15%-67.54%. The polysaccharide of H.scandens had antioxidant activities on O</sup>-2·and ·OH.
Key words:  Humulus scandens  Polysaccharide  antioxidant activity
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